The aim of the project is to develop a method for genetic diagnosis of Salmonella bacteria that does not require a full procedure for isolation of genetic material from a sample, together with preliminary validation of a Salmonella diagnostic kit based on genetic identification by Real-Time Loop Mediated Isothermal Amplification (LAMP).
The duration of the project has been scheduled for 10 months and will include the following stages:
Work on developing a set of buffers for sample storage and/or accelerated isolation of genetic material.
Work on reducing the amplification time of genetic material in the Direct test to less than 15 min.
Work on analyzing the compatibility of the diagnostic kit with leading Real-Time PCR thermocyclers.
The described R&D Project will be carried out in the PoC phase.
R&D activities will be carried out in Poland; their results will be subject to protection, among others, in the territory of Poland.
